Human Carbonyl Reductase Nadph 1, CBR1 ELISA Kit

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Human Carbonyl Reductase Nadph 1, CBR1 ELISA Kit

Human Carbonyl Reductase Nadph 1, CBR1 ELISA Kit

SKU:
ABM218376
Manufacturer SKU
E6599Hu
Product type
ELISA Kits
TargetCBR1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol (PubMed:18449627, PubMed:15799708, PubMed:17912391, PubMed:7005231). Can convert prostaglandin E to prostaglandin F2-alpha (By similarity). Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione (PubMed:18826943, PubMed:17344335).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CBR1 antibody. CBR1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CBR1 Antibody is added and binds to CBR1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CBR1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CBR1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Cell Biology, Metabolism
Also known as
20-beta-hydroxysteroid dehydrogenase;Carbonyl reductase [NADPH] 1;CBR;CBR1;CRN;hCBR1;NADPH-dependent carbonyl reductase 1;PG-9-KR;Prostaglandin 9-ketoreductase;Prostaglandin-E(2 9-reductase;SDR21C1;Short chain dehydrogenase/reductase family 21C member 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.31ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
30,375 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6599Hu
Gene symbol
CBR1
Gene ID
873
UniProt accession
P16152
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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