Manufactured by:BT LAB
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Human Carbonic Anhydrase 7, CA7 ELISA Kit

Human Carbonic Anhydrase 7, CA7 ELISA Kit

SKU:
ABM218374
Manufacturer SKU
E6598Hu
Product type
ELISA Kits
TargetCA7
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Carbonic anhydrase 7 (CA7) is an enzyme that in humans is encoded by the CA7 gene. Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CA7 antibody. CA7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CA7 Antibody is added and binds to CA7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CA7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CA7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Tags & Cell Markers, Cell Biology
Also known as
CA7;Carbonate dehydratase VII;Carbonic anhydrase 7;Carbonic anhydrase VII;CAVII;CA-VII
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
33.05ng/L
Detection range
70-15000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
29,658 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6598Hu
Gene symbol
CA7
Gene ID
766
UniProt accession
P43166
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00