Human Beta-defensin 128, DEFB128 ELISA Kit

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Human Beta-defensin 128, DEFB128 ELISA Kit

Human Beta-defensin 128, DEFB128 ELISA Kit

SKU:
ABM218300
Manufacturer SKU
E6550Hu
Product type
ELISA Kits
TargetDEFB128
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Product details

Target
DEFB128
Assay type
Sandwich
Reactivity
Human
Sample types
Serum; Plasma; Cell culture supernatants; Ascites; Tissue homogenates
Detection range
0.05-20ng/ml

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human DEFB128 antibody. DEFB128 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DEFB128 Antibody is added and binds to DEFB128 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DEFB128 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DEFB128. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Microbiology, Immunology
Also known as
Beta-defensin 128;Beta-defensin 28;DEFB128;DEFB28;DEFB-28;Defensin, beta 128;hBD-28
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.024ng/ml
Detection range
0.05-20ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
10,650 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6550Hu
Gene symbol
DEFB128
Gene ID
245939
UniProt accession
Q7Z7B8
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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