Human Transcription Factor 3B 50 kDa Subunit, BRF2 ELISA Kit

BT LAB manufacturer product image

100%
Manufactured by:BT LAB
Save 15%RUO

Human Transcription Factor 3B 50 kDa Subunit, BRF2 ELISA Kit

Human Transcription Factor 3B 50 kDa Subunit, BRF2 ELISA Kit

SKU:
ABM217950
Manufacturer SKU
E6289Hu
Product type
ELISA Kits
TargetBRF2
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (PubMed:11040218, PubMed:11121026, PubMed:11564744, PubMed:26638071). Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress (PubMed:26638071). Down-regulates expression of target genes in response to oxidative stress (PubMed:26638071). Overexpression protects cells against apoptosis in response to oxidative stress (PubMed:26638071).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human BRF2 antibody. BRF2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human BRF2 Antibody is added and binds to BRF2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BRF2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human BRF2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Epigenetics and Nuclear Signaling
Also known as
B-related factor 2;BRF2;BRF-2;BRFU;hBRFU;hTFIIIB50;TFIIIB50;Transcription factor IIIB 50 kDa subunit
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.75ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
46,533 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6289Hu
Gene symbol
BRF2
Gene ID
55290
UniProt accession
Q9HAW0
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00