Human Serine Threonine-protein Phosphatase 2A 65 kDa Regulatory Subunit A Alpha Isoform, PPP2R1A ELISA Kit

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Human Serine Threonine-protein Phosphatase 2A 65 kDa Regulatory Subunit A Alpha Isoform, PPP2R1A ELISA Kit

Human Serine Threonine-protein Phosphatase 2A 65 kDa Regulatory Subunit A Alpha Isoform, PPP2R1A ELISA Kit

SKU:
ABM217726
Manufacturer SKU
E6152Hu
Product type
ELISA Kits
TargetPPP2R1A
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PPP2R1A antibody. PPP2R1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PPP2R1A Antibody is added and binds to PPP2R1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPP2R1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PPP2R1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Medium tumor antigen-associated 61 kDa protein;MRD36;PP2A subunit A isoform PR65-alpha;PP2A subunit A isoform R1-alpha;PP2AA;PP2AAALPHA;PP2A-Aalpha;PPP2R1A;PR65A;Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.02ng/L
Detection range
20-3500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
65,309 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6152Hu
Gene symbol
PPP2R1A
Gene ID
5518
UniProt accession
P30153
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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