Human Glutathione Peroxidase 6, GPX6 ELISA Kit

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Human Glutathione Peroxidase 6, GPX6 ELISA Kit

Human Glutathione Peroxidase 6, GPX6 ELISA Kit

SKU:
ABM216516
Manufacturer SKU
E5344Hu
Product type
ELISA Kits
TargetGPX6
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Glutathione peroxidase 6 (GPx-6) is an enzyme that in humans is encoded by the GPX6 gene. This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Expression of this gene is restricted to embryos and adult olfactory epithelium.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GPX6 antibody. GPX6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GPX6 Antibody is added and binds to GPX6 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GPX6 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GPX6. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
dJ1186N24;dJ1186N24.1;Glutathione peroxidase 6;GPX5p;GPX6;GPx-6;GPXP3;GSHPx-6
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.33mU/mL
Detection range
0.5-200mU/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
24,971 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5344Hu
Gene symbol
GPX6
Gene ID
257202
UniProt accession
P59796
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00