Human E3 Ubiquitin-protein Ligase Mylip, MYLIP ELISA Kit

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Human E3 Ubiquitin-protein Ligase Mylip, MYLIP ELISA Kit

Human E3 Ubiquitin-protein Ligase Mylip, MYLIP ELISA Kit

SKU:
ABM216302
Manufacturer SKU
E5215Hu
Product type
ELISA Kits
TargetMYLIP
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Myosin regulatory light chain interacting protein, also known as MYLIP, is a protein that in humans is encoded by the MYLIP gene. MYLIP is also known as IDOL "Inducible Degrader of the LDL receptor" based on its involvement in cholesterol regulation or MIR "Modulator Of Immune Recognition". The expression of IDOL is induced by the sterol-activated liver X receptor.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MYLIP antibody. MYLIP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MYLIP Antibody is added and binds to MYLIP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MYLIP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MYLIP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
E3 ubiquitin-protein ligase MYLIP;IDOL;Inducible degrader of the LDL-receptor;MIR;MYLIP;Myosin regulatory light chain interacting protein;RING-type E3 ubiquitin transferase MYLIP
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.24ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
49,910 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5215Hu
Gene symbol
MYLIP
Gene ID
29116
UniProt accession
Q8WY64
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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