Manufactured by:BT LAB
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Human Interleukin 12 Receptor Subunit Beta-1, IL12RB1 ELISA Kit

Human Interleukin 12 Receptor Subunit Beta-1, IL12RB1 ELISA Kit

SKU:
ABM215226
Manufacturer SKU
E3978Hu
Product type
ELISA Kits
TargetIL12RB1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL12RB1 antibody. IL12RB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL12RB1 Antibody is added and binds to IL12RB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL12RB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL12RB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
CD antigen CD212;IL 12RB1;IL-12 receptor beta component;IL-12 receptor subunit beta-1;IL-12R subunit beta-1;IL12RB1;IL-12RB1;IL-12R-beta-1;Interleukin-12 receptor subunit beta-1;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.01ng/ml
Detection range
0.05-15ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
73,109 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3978Hu
Gene symbol
IL12RB1
Gene ID
3594
UniProt accession
P42701
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL12RB1interleukin 12 receptor subunit beta 1

View target guide

IL12RB1 identifies interleukin 12 receptor subunit beta 1 in Homo sapiens (Human). This target record connects NCBI Gene 3594, UniProtKB P42701 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
3594
UniProtKB
P42701
BT LAB
£240.93£205.00