Human Ubiquitin Carboxyl Terminalhydrolase Bap1, BAP1 ELISA Kit

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Human Ubiquitin Carboxyl Terminalhydrolase Bap1, BAP1 ELISA Kit

Human Ubiquitin Carboxyl Terminalhydrolase Bap1, BAP1 ELISA Kit

SKU:
ABM215174
Manufacturer SKU
E3920Hu
Product type
ELISA Kits
TargetBAP1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) is a deubiquitinating enzyme that in humans is encoded by the BAP1 gene. BAP1 encodes an 80.4 kDa nuclear-localizing protein with a ubiquitin carboxy-terminal hydrolase (UCH) domain that gives BAP1 its deubiquitinase activity. Recent studies have shown that BAP1 and its fruit fly homolog, Calypso, are members of the polycomb-group proteins (PcG) of highly conserved transcriptional repressors required for long-term silencing of genes that regulate cell fate determination, stem cell pluripotency, and other developmental processes.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human BAP1 antibody. BAP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human BAP1 Antibody is added and binds to BAP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BAP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human BAP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Epigenetics and Nuclear Signaling
Also known as
BAP1;BRCA1-associated protein 1;Cerebral protein 6;HUCEP-13;hucep-6;Ubiquitin carboxyl-terminal hydrolase BAP1;UCHL2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.91ng/ml
Detection range
20-7000ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
80,362 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3920Hu
Gene symbol
BAP1
Gene ID
8314
UniProt accession
Q92560
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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