Manufactured by:BT LAB
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Human High Sensitive Interleukin 2, HS IL-2 ELISA Kit

Human High Sensitive Interleukin 2, HS IL-2 ELISA Kit

SKU:
ABM215054
Manufacturer SKU
E3761Hu
Product type
ELISA Kits
TargetIL2
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL2 antibody. IL2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL2 Antibody is added and binds to IL2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
Aldesleukin;IL 2;IL2;IL-2;Interleukin-2;T-cell growth factor;TCGF
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.23ng/L
Detection range
0.5-200ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
17,628 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3761Hu
Gene symbol
IL2
Gene ID
3558
UniProt accession
P60568
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL2interleukin 2

View target guide

IL2 identifies interleukin 2 in Homo sapiens (Human). This target record connects NCBI Gene 3558, UniProtKB P60568 and related ABMIUM catalogue products.

Biological function: IL2 is a cytokine produced mainly by activated T cells that supports immune-cell proliferation, differentiation and survival. It signals through IL2 receptor complexes to activate JAK-STAT, PI3K and MAPK pathways, supporting T-cell, regulatory T-cell, NK-cell and B-cell responses.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3558
UniProtKB
P60568
BT LAB
£240.93£205.00