Manufactured by:BT LAB
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Human Succinate Dehydrogenase Iron-sulfur Subunit, Mitochondrial, SDHB ELISA Kit

Human Succinate Dehydrogenase Iron-sulfur Subunit, Mitochondrial, SDHB ELISA Kit

SKU:
ABM215026
Manufacturer SKU
E3726Hu
Product type
ELISA Kits
TargetSDHB
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SDHB antibody. SDHB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SDHB Antibody is added and binds to SDHB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SDHB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SDHB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
CWS2;IP;Iron-sulfur subunit of complex II;MC2DN4;PGL4;SDH;SDH1;SDH2;SDHB;Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.027ng/ml
Detection range
0.05-30ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
31,630 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3726Hu
Gene symbol
SDHB
Gene ID
6390
UniProt accession
P21912
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SDHBsuccinate dehydrogenase complex iron sulfur subunit B

View target guide

SDHB identifies succinate dehydrogenase complex iron sulfur subunit B in Homo sapiens (Human). This target record connects NCBI Gene 6390, UniProtKB P21912 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6390
UniProtKB
P21912
BT LAB
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