Manufactured by:BT LAB
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Human Fibroblast Growth Factor 4, FGF4 ELISA Kit

Human Fibroblast Growth Factor 4, FGF4 ELISA Kit

SKU:
ABM214786
Manufacturer SKU
E3414Hu
Product type
ELISA Kits
TargetFGF4
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human FGF4 antibody. FGF4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FGF4 Antibody is added and binds to FGF4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FGF4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human FGF4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular, Signal Transduction, Cancer
Also known as
FGF 4;FGF4;FGF-4;Fibroblast growth factor 4;HBGF-4;Heparin secretory-transforming protein 1;Heparin-binding growth factor 4;HST;HST-1;HSTF-1;Transforming protein KS3
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.52ng/L
Detection range
1-400ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
22,048 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3414Hu
Gene symbol
FGF4
Gene ID
2249
UniProt accession
P08620
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FGF4fibroblast growth factor 4

View target guide

FGF4 identifies fibroblast growth factor 4 in Homo sapiens (Human). This target record connects NCBI Gene 2249, UniProtKB P08620 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2249
UniProtKB
P08620
BT LAB
£240.93£205.00