Manufactured by:BT LAB
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Human Annexin A3, ANXA3 ELISA Kit

Human Annexin A3, ANXA3 ELISA Kit

SKU:
ABM214650
Manufacturer SKU
E3289Hu
Product type
ELISA Kits
TargetANXA3
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ANXA3 antibody. ANXA3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ANXA3 Antibody is added and binds to ANXA3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ANXA3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ANXA3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
35-alpha calcimedin;Annexin A3;Annexin III;Annexin-3;ANX3;ANXA3;Inositol 1,2-cyclic phosphate 2-phosphohydrolase;Lipocortin III;PAP-III;Placental anticoagulant protein III
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.055ng/ml
Detection range
0.1-35ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
36,375 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3289Hu
Gene symbol
ANXA3
Gene ID
306
UniProt accession
P12429
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ANXA3annexin A3

View target guide

Annexin A3 (ANXA3) is a calcium- and phospholipid-binding annexin family protein associated with membrane biology and phospholipid-dependent enzymatic regulation.

Biological function: Reviewed UniProt P12429 describes ANXA3 as an inhibitor of phospholipase A2 with anticoagulant properties and inositol 1,2-cyclic phosphate 2-phosphohydrolase activity. These functions place annexin A3 in calcium/phospholipid-dependent membrane and signalling biology.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
306
UniProtKB
P12429
BT LAB
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