Manufactured by:BT LAB
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Human Tumor Necrosis Factor Ligand Superfamily Member 10, TNFSF10 ELISA Kit

Human Tumor Necrosis Factor Ligand Superfamily Member 10, TNFSF10 ELISA Kit

SKU:
ABM214464
Manufacturer SKU
E3154Hu
Product type
ELISA Kits
TargetTNFSF10
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed: 26457518, PubMed: 10549288). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TNFSF10 antibody. TNFSF10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TNFSF10 Antibody is added and binds to TNFSF10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TNFSF10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TNFSF10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cancer
Also known as
Apo-2 ligand;Apo-2L;CD antigen CD253;Protein TRAIL;TNF-related apoptosis-inducing ligand;TNFSF 10;TNFSF10;TRAIL;Tumor necrosis factor ligand superfamily member 10;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.49ng/L
Detection range
5-1500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
32,509 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3154Hu
Gene symbol
TNFSF10
Gene ID
8743
UniProt accession
P50591
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

TNFSF10TNF superfamily member 10

View target guide

TNFSF10 identifies TNF superfamily member 10 in Homo sapiens (Human). This target record connects NCBI Gene 8743, UniProtKB P50591 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
8743
UniProtKB
P50591
BT LAB
£240.93£205.00