Manufactured by:BT LAB
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Human Cell Surface A33 Antigen, GPA33 ELISA Kit

Human Cell Surface A33 Antigen, GPA33 ELISA Kit

SKU:
ABM213948
Manufacturer SKU
E2721Hu
Product type
ELISA Kits
TargetGPA33
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cell surface A33 antigen is a protein that in humans is encoded by the GPA33 gene. The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GPA33 antibody. GPA33 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GPA33 Antibody is added and binds to GPA33 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GPA33 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GPA33. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
A33;Cell surface A33 antigen;Glycoprotein A33;GPA33
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.95ng/L
Detection range
20-3800ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
35,632 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2721Hu
Gene symbol
GPA33
Gene ID
10223
UniProt accession
Q99795
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GPA33glycoprotein A33

View target guide

GPA33 identifies glycoprotein A33 in Homo sapiens (Human). This target record connects NCBI Gene 10223, UniProtKB Q99795 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
10223
UniProtKB
Q99795
BT LAB
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