Manufactured by:BT LAB
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Mouse Glutathione Peroxidase 1, GPX1 ELISA Kit

Mouse Glutathione Peroxidase 1, GPX1 ELISA Kit

SKU:
ABM213658
Manufacturer SKU
E2594Mo
Product type
ELISA Kits
TargetGPX1
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism (PubMed: 9195979).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse GPX1 antibody. GPX1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse GPX1 Antibody is added and binds to GPX1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GPX1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse GPX1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Metabolism
Also known as
Cellular glutathione peroxidase;Glutathione peroxidase 1;GPX 1;GPX1;GPx-1;GSHPx-1;Selenium-dependent glutathione peroxidase 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.33ng/ml
Detection range
0.5-200ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
22,329 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2594Mo
Gene symbol
GPX1
Gene ID
14775
UniProt accession
P11352
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GPX1glutathione peroxidase 1

View target guide

GPX1 identifies glutathione peroxidase 1 in Homo sapiens (Human). This target record connects NCBI Gene 2876, UniProtKB P07203 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2876
UniProtKB
P07203
BT LAB
£240.93£205.00