Mouse Tyrosine-protein Phosphatase Non-receptor Type 4, PTPN4 ELISA Kit

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Mouse Tyrosine-protein Phosphatase Non-receptor Type 4, PTPN4 ELISA Kit

Mouse Tyrosine-protein Phosphatase Non-receptor Type 4, PTPN4 ELISA Kit

SKU:
ABM213526
Manufacturer SKU
E2548Mo
Product type
ELISA Kits
TargetPTPN4
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Phosphatase that plays a role in immunity, learning, synaptic plasticity or cell homeostasis (PubMed: 17953619, PubMed: 25825441). Regulates neuronal cell homeostasis by protecting neurons against apoptosis (By similarity). Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction (PubMed: 25825441). Dephosphorylates also the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway (PubMed: 18614237). May act at junctions between the membrane and the cytoskeleton.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse PTPN4 antibody. PTPN4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PTPN4 Antibody is added and binds to PTPN4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PTPN4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PTPN4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
PTPN 4;PTPN4;Testis-enriched protein tyrosine phosphatase;Tyrosine-protein phosphatase non-receptor type 4
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.27ng/L
Detection range
2-600ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
105,832 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2548Mo
Gene symbol
PTPN4
Gene ID
19258
UniProt accession
Q9WU22
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00