Human Mitogen Activated Protein Kinase 1, MAP3K1 ELISA Kit

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Human Mitogen Activated Protein Kinase 1, MAP3K1 ELISA Kit

Human Mitogen Activated Protein Kinase 1, MAP3K1 ELISA Kit

SKU:
ABM213348
Manufacturer SKU
E2492Hu
Product type
ELISA Kits
TargetMAP3K1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Component of a protein kinase signal transduction cascade (PubMed: 9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed: 9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed: 17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed: 9808624).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MAP3K1 antibody. MAP3K1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MAP3K1 Antibody is added and binds to MAP3K1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MAP3K1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MAP3K1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
MAP3K1;MAPK/ERK kinase 1;MEK kinase 1;MEKK 1;Mitogen-activated protein kinase kinase 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.55ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
164,470 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2492Hu
Gene symbol
MAP3K1
Gene ID
4214
UniProt accession
Q13233
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

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