Human Copine 1, CPNE1 ELISA Kit

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Human Copine 1, CPNE1 ELISA Kit

Human Copine 1, CPNE1 ELISA Kit

SKU:
ABM213172
Manufacturer SKU
E2437Hu
Product type
ELISA Kits
TargetCPNE1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes (PubMed: 14674885). Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner (PubMed: 14674885). Exhibits calcium-dependent phospholipid binding properties (PubMed: 9430674, PubMed: 19539605). Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner (PubMed: 23263657, PubMed: 25450385). May recruit target proteins to the cell membrane in a calcium-dependent manner (PubMed: 12522145). May function in membrane trafficking (PubMed: 9430674). Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit (PubMed: 18212740). Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL (PubMed: 18212740).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CPNE1 antibody. CPNE1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CPNE1 Antibody is added and binds to CPNE1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CPNE1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CPNE1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Chromobindin 17;Copine I;Copine-1;CPNE 1;CPNE1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.027ng/ml
Detection range
0.05-10ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
59,059 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2437Hu
Gene symbol
CPNE1
Gene ID
8904
UniProt accession
Q99829
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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