Manufactured by:BT LAB
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Bovine Beta-1, B4GALT1 ELISA Kit

Bovine Beta-1, B4GALT1 ELISA Kit

SKU:
ABM212096
Manufacturer SKU
E2188Bo
Product type
ELISA Kits
TargetB4GALT1
ApplicationsELISA
Species / reactivityBovine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. The secreted form is responsible for the synthesis of complex-type to N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids (PubMed:9405390).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine B4GALT1 antibody. B4GALT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine B4GALT1 Antibody is added and binds to B4GALT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated B4GALT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine B4GALT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
B4GALT1;b4Gal-T1;Beta-1,4-galactosyltransferase 1;Beta-1,4-GalTase 1;Beta4Gal-T1;Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase;Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase;GGTB2;Lactose Synthase A protein;N-acetyllactosamine Synthase;Nal Synthase;Neolactotriaosylceramide beta-1,4-galactosyltransferase;Processed beta-1,4-galactosyltransferase 1];UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.029ng/mL
Detection range
0.05-20ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
44,843 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2188Bo
Gene symbol
B4GALT1
Gene ID
281781
UniProt accession
P08037
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00