Manufactured by:BT LAB
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Mouse Interleukin-12 Receptor Subunit Beta-2, IL12RB2 ELISA Kit

Mouse Interleukin-12 Receptor Subunit Beta-2, IL12RB2 ELISA Kit

SKU:
ABM212034
Manufacturer SKU
E2174Mo
Product type
ELISA Kits
TargetIL12RB2
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Can also activate STAT3.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse IL12RB2 antibody. IL12RB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL12RB2 Antibody is added and binds to IL12RB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL12RB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IL12RB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
IL 12RB2;IL-12 receptor subunit beta-2;IL-12R subunit beta-2;IL12RB2;IL-12RB2;IL-12R-beta-2;Interleukin-12 receptor subunit beta-2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.027ng/mL
Detection range
0.05-20ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
98,197 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2174Mo
Gene symbol
IL12RB2
Gene ID
16162
UniProt accession
P97378
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00