Mouse Interferon Regulatory Factor 4, IRF4 ELISA Kit

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Mouse Interferon Regulatory Factor 4, IRF4 ELISA Kit

Mouse Interferon Regulatory Factor 4, IRF4 ELISA Kit

SKU:
ABM212000
Manufacturer SKU
E2162Mo
Product type
ELISA Kits
TargetIRF4
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse IRF4 antibody. IRF4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IRF4 Antibody is added and binds to IRF4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IRF4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IRF4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Interferon regulatory factor 4;IRF 4;IRF4;IRF-4;LSIRF;Lymphocyte-specific interferon regulatory factor;NF-EM5;PU.1 interaction partner;Transcriptional activator PIP;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.34ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
51,577 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2162Mo
Gene symbol
IRF4
Gene ID
16364
UniProt accession
Q64287
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00