Rat Multidrug Resistance-associated Protein 1, ABCC1 ELISA Kit

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Rat Multidrug Resistance-associated Protein 1, ABCC1 ELISA Kit

Rat Multidrug Resistance-associated Protein 1, ABCC1 ELISA Kit

SKU:
ABM211972
Manufacturer SKU
E2156Ra
Product type
ELISA Kits
TargetABCC1
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency (PubMed: 15129170). Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation (PubMed: 17050692). Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ABCC1 antibody. ABCC1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ABCC1 Antibody is added and binds to ABCC1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCC1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ABCC1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Cancer, Cardiovascular, Metabolism
Also known as
ABCC 1;ABCC1;ATP-binding cassette sub-family C member 1;Glutathione-S-conjugate-translocating ATPase ABCC1;Leukotriene C(4 transporter;LTC4 transporter;Multidrug resistance-associated protein 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.45ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
171,493 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2156Ra
Gene symbol
ABCC1
Gene ID
24565
UniProt accession
Q8CG09
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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