Manufactured by:BT LAB
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Mouse Beta-defensin 2, DEFB2 ELISA Kit

Mouse Beta-defensin 2, DEFB2 ELISA Kit

SKU:
ABM210762
Manufacturer SKU
E1738Mo
Product type
ELISA Kits
TargetDEFB2
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Beta-defensin 2 (BD-2) also known as skin-antimicrobial peptide 1 (SAP1) is a peptide that in humans is encoded by the DEFB4 (defensin, beta 4) gene. Human beta-defensin-2 (hBD-2) is a cysteine-rich cationic low molecular weight antimicrobial peptide recently discovered in lesional skin.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse DEFB2 antibody. DEFB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse DEFB2 Antibody is added and binds to DEFB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DEFB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse DEFB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Microbiology
Also known as
BD-2;Beta-defensin 2;DEFB2;Defensin, beta 2;mBD-2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.52ng/L
Detection range
5-1000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
7,820 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1738Mo
Gene symbol
Defb2
Gene ID
13215
UniProt accession
P82020
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

DEFB4ADefensin beta 4A

View target guide

DEFB4A is associated with Defensin beta 4A in Homo sapiens (Human). This target record connects UniProtKB O15263 with related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
UniProtKB
O15263
BT LAB
£240.93£205.00