Manufactured by:BT LAB
Save 15%RUO

Rat Gtpase Hras, HRAS ELISA Kit

Rat Gtpase Hras, HRAS ELISA Kit

SKU:
ABM210760
Manufacturer SKU
E1736Ra
Product type
ELISA Kits
TargetHRas
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

GTPase HRas, from "Harvey Rat sarcoma virus", also known as transforming protein p21 is an enzyme that in humans is encoded by the HRAS gene. The HRAS gene is located on the short (p) arm of chromosome 11 at position 15.5, from base pair 522,241 to base pair 525,549. HRas is a small G protein in the Ras subfamily of the Ras superfamily of small GTPases. Once bound to Guanosine triphosphate, H-Ras will activate a Raf kinase like c-Raf, the next step in the MAPK/ERK pathway.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HRas antibody. HRas present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HRas Antibody is added and binds to HRas in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HRas antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HRas. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
c-H-ras;GTPase HRas;GTPase HRas, N-terminally processed];HRas;HRAS1;H-Ras-1;p21ras;Transforming protein p21
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
3.65ng/L
Detection range
6-1500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
21,298 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1736Ra
Gene symbol
Hras
Gene ID
293621
UniProt accession
P20171
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

HRASHRas proto-oncogene, GTPase

View target guide

HRAS identifies HRas proto-oncogene, GTPase in Homo sapiens (Human). This target record connects NCBI Gene 3265, UniProtKB P01112 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
3265
UniProtKB
P01112
BT LAB
£240.93£205.00