Manufactured by:BT LAB
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Mouse Adenosine Deaminase, ADA ELISA Kit

Mouse Adenosine Deaminase, ADA ELISA Kit

SKU:
ABM210576
Manufacturer SKU
E1600Mo
Product type
ELISA Kits
TargetADA
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Adenosine deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme (EC 3.5.4.4) involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. Its primary function in humans is the development and maintenance of the immune system. However, the full physiological role of ADA is not yet completely understood.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ADA antibody. ADA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ADA Antibody is added and binds to ADA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ADA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Epigenetics and Nuclear Signaling
Also known as
ADA;Adenosine aminohydrolase;Adenosine deaminase
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.023ng/ml
Detection range
0.05-30ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
39,992 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1600Mo
Gene symbol
Ada
Gene ID
11486
UniProt accession
P03958
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ADAadenosine deaminase

View target guide

Adenosine deaminase (ADA), also known as ADA1, is an enzyme of purine metabolism that deaminates adenosine and 2-deoxyadenosine. This ABMIUM target record links ADA biology, verified human identifiers and catalogue-backed research products.

Biological function: ADA catalyzes hydrolytic deamination of adenosine to inosine and 2-deoxyadenosine to deoxyinosine, helping regulate purine metabolism and adenosine homeostasis. Reviewed UniProt annotation also describes extracellular signalling roles associated with cell-surface ADA.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
100
UniProtKB
P00813
BT LAB
£240.93£205.00