Mouse Atrial Natriuretic Peptide Receptor 3, NPR3 ELISA Kit

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Mouse Atrial Natriuretic Peptide Receptor 3, NPR3 ELISA Kit

Mouse Atrial Natriuretic Peptide Receptor 3, NPR3 ELISA Kit

SKU:
ABM209962
Manufacturer SKU
E1231Mo
Product type
ELISA Kits
TargetNPR3
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor for the natriuretic Peptide hormones, binding with similar affinities atrial natriuretic Peptide NPPA/ANP, brain natriuretic Peptide NPPB/BNP, and C-type natriuretic Peptide NPPC/CNP (PubMed: 10377427, PubMed: 17951249). May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects (PubMed: 10377427, PubMed: 17951249). May regulate diuresis, blood pressure and skeletal development (PubMed: 10377427). Does not have guanylate cyclase activity (PubMed: 10377427).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse NPR3 antibody. NPR3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NPR3 Antibody is added and binds to NPR3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NPR3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse NPR3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
ANP-C;ANPR-C;Atrial natriuretic Peptide clearance receptor;Atrial natriuretic Peptide receptor 3;Atrial natriuretic Peptide receptor type C;EF-2;NPR 3;NPR3;NPR-C;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.54ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
59,808 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1231Mo
Gene symbol
NPR3
Gene ID
18162
UniProt accession
P70180
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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