Manufactured by:BT LAB
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Rat Cathepsin B, CATH-B ELISA Kit

Rat Cathepsin B, CATH-B ELISA Kit

SKU:
ABM209374
Manufacturer SKU
E0871Ra
Product type
ELISA Kits
Targetcath-B
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Cathepsin B belongs to a family of lysosomal cysteine proteases and plays an important role in intracellular proteolysis. In humans, cathepsin B is encoded by the CTSB gene. Cathepsin B is upregulated in certain cancers, in pre-malignant lesions, and in various other pathological conditions.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat cath-B antibody. cath-B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat cath-B Antibody is added and binds to cath-B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated cath-B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat cath-B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
cath-B;Cathepsin B;Cathepsin B heavy chain];Cathepsin B light chain;Cathepsin B1;RSG-2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.11ng/ml
Detection range
0.2-70ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
37,470 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0871Ra
Gene symbol
Ctsb
Gene ID
64529
UniProt accession
P00787
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CTSBcathepsin B

View target guide

CTSB identifies cathepsin B in Homo sapiens (Human). This target record connects NCBI Gene 1508, UniProtKB P07858 and related ABMIUM catalogue products.

Biological function: CTSB (cathepsin B) is a lysosomal cysteine protease with endopeptidase and exopeptidase activity that contributes to intracellular protein turnover and proteolytic processing.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1508
UniProtKB
P07858
BT LAB
£240.93£205.00