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Rat Eosinophil Cationic Protein, ECP ELISA Kit

Rat Eosinophil Cationic Protein, ECP ELISA Kit

SKU:
ABM208868
Manufacturer SKU
E0560Ra
Product type
ELISA Kits
TargetRNASE3
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat RNASE3 antibody. RNASE3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat RNASE3 Antibody is added and binds to RNASE3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RNASE3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat RNASE3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
EAR-11;ECP;Eosinophil cationic protein;Eosinophil secondary granule ribonuclease 11;Ribonuclease 3;RNase 3;RNASE3
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
14.57ng/L
Detection range
30-9000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
18,007 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0560Ra
Gene symbol
RNASE3
UniProt accession
P70709
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

RNASE3ribonuclease A family member 3

View target guide

RNASE3 identifies ribonuclease A family member 3 in Homo sapiens (Human). This target record connects NCBI Gene 6037, UniProtKB P12724 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
6037
UniProtKB
P12724
BT LAB
£240.93£205.00