Manufactured by:BT LAB
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Porcine Peroxisome Proliferation Activated Receptor Gamma, PPARG ELISA Kit

Porcine Peroxisome Proliferation Activated Receptor Gamma, PPARG ELISA Kit

SKU:
ABM208726
Manufacturer SKU
E0456Po
Product type
ELISA Kits
TargetPPARG
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine PPARG antibody. PPARG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine PPARG Antibody is added and binds to PPARG in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPARG antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine PPARG. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Nuclear receptor subfamily 1 group C member 3;Peroxisome proliferator-activated receptor gamma;PPAR G;PPARG;PPAR-gamma
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.02ng/ml
Detection range
0.05-20ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
57,512 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0456Po
Gene symbol
PPARG
Gene ID
397671
UniProt accession
O62807
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PPARGperoxisome proliferator activated receptor gamma

View target guide

PPARG identifies peroxisome proliferator activated receptor gamma in Homo sapiens (Human). This target record connects NCBI Gene 5468, UniProtKB P37231 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5468
UniProtKB
P37231
BT LAB
£240.93£205.00