Manufactured by:BT LAB
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Canine Calcitonin Gene Related Peptide, CGRP ELISA Kit

Canine Calcitonin Gene Related Peptide, CGRP ELISA Kit

SKU:
ABM208690
Manufacturer SKU
E0435Ca
Product type
ELISA Kits
TargetCALCA
ApplicationsELISA
Species / reactivityCanine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine CALCA antibody. CALCA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine CALCA Antibody is added and binds to CALCA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CALCA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine CALCA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Alpha-type CGRP;CALCA;Calcitonin gene-related Peptide 1;Calcitonin gene-related Peptide I;CGRP-I
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
4.78ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
13,874 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0435Ca
Gene symbol
CALCA
Gene ID
403946
UniProt accession
Q9MYV1
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CALCAcalcitonin related polypeptide alpha

View target guide

CALCA identifies calcitonin related polypeptide alpha in Homo sapiens (Human). This target record connects NCBI Gene 796, UniProtKB P01258 and related ABMIUM catalogue products.

Biological function: CALCA encodes calcitonin-family peptide products. Calcitonin is a peptide hormone that lowers circulating calcium and phosphate by promoting their incorporation into bone and signals through calcitonin receptor complexes.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
796
UniProtKB
P01258
BT LAB
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