Manufactured by:BT LAB
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Human Insulin-like Growth Factors Binding Protein 4, IGFBP4 ELISA Kit

Human Insulin-like Growth Factors Binding Protein 4, IGFBP4 ELISA Kit

SKU:
ABM208640
Manufacturer SKU
E0406Hu
Product type
ELISA Kits
TargetIGFBP4
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IGFBP4 antibody. IGFBP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IGFBP4 Antibody is added and binds to IGFBP4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IGFBP4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IGFBP4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
IBP-4;IGF-binding protein 4;IGFBP 4;IGFBP4;IGFBP-4;Insulin-like growth factor-binding protein 4
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
14.54ng/mL
Detection range
30-9000ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
27,934 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0406Hu
Gene symbol
IGFBP4
Gene ID
3487
UniProt accession
P22692
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IGFBP4insulin like growth factor binding protein 4

View target guide

IGFBP4 identifies insulin like growth factor binding protein 4 in Homo sapiens (Human). This target record connects NCBI Gene 3487, UniProtKB P22692 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3487
UniProtKB
P22692
BT LAB
£344.83£293.00