Manufactured by:BT LAB
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Porcine Prolactin, PRL ELISA Kit

Porcine Prolactin, PRL ELISA Kit

SKU:
ABM207990
Manufacturer SKU
E0194Po
Product type
ELISA Kits
TargetPRL
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Prolactin acts primarily on the mammary gland by promoting lactation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine PRL antibody. PRL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine PRL Antibody is added and binds to PRL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PRL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine PRL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
PRL;Prolactin
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.152ng/L
Detection range
0.3-90ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
26,141 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0194Po
Gene symbol
PRL
Gene ID
396965
UniProt accession
P01238
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PRLprolactin

View target guide

PRL identifies prolactin in Homo sapiens (Human). This target record connects NCBI Gene 5617, UniProtKB P01236 and related ABMIUM catalogue products.

Biological function: PRL (prolactin) is a pituitary peptide hormone with central roles in mammary-gland development and milk production. It also has broader endocrine, reproductive and immune-associated biological effects mediated through the prolactin receptor.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5617
UniProtKB
P01236
BT LAB
£240.93£205.00