Manufactured by:BT LAB
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Human Intelectin-1, ITLN1 ELISA Kit

Human Intelectin-1, ITLN1 ELISA Kit

SKU:
ABM207892
Manufacturer SKU
E0155Hu
Product type
ELISA Kits
TargetITLN1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed: 11313366, PubMed: 26148048). Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) (PubMed: 26148048). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris (PubMed: 26148048). Does not bind human glycans (PubMed: 26148048). Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes (PubMed: 16531507). Increases AKT phosphorylation in the absence and presence of insulin (PubMed: 16531507). May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (PubMed: 11747454, PubMed: 23921499).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ITLN1 antibody. ITLN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ITLN1 Antibody is added and binds to ITLN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ITLN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ITLN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
Endothelial lectin HL-1;Galactofuranose-binding lectin;Intelectin-1;Intestinal lactoferrin receptor;ITLN 1;ITLN1;ITLN-1;Omentin
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.03ng/L
Detection range
2-600ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
34,962 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0155Hu
Gene symbol
ITLN1
Gene ID
55600
UniProt accession
Q8WWA0
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£344.83£293.00