Manufactured by:BT LAB
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Bovine Pulmonary Surfatcant-associated Protein D, SP-D ELISA Kit

Bovine Pulmonary Surfatcant-associated Protein D, SP-D ELISA Kit

SKU:
ABM207866
Manufacturer SKU
E0149Bo
Product type
ELISA Kits
TargetSFTPD
ApplicationsELISA
Species / reactivityBovine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine SFTPD antibody. SFTPD present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine SFTPD Antibody is added and binds to SFTPD in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFTPD antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine SFTPD. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Cancer, Microbiology
Also known as
Lung surfactant protein D;PSP D;PSP-D;Pulmonary surfactant-associated protein D;SFTPD;SP D;SP-D
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.31ng/ml
Detection range
0.5-200ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
37,405 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0149Bo
Gene symbol
SFTPD
Gene ID
282072
UniProt accession
P35246
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SFTPDsurfactant protein D

View target guide

SFTPD identifies surfactant protein D in Homo sapiens (Human). This target record connects NCBI Gene 6441, UniProtKB P35247 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6441
UniProtKB
P35247
BT LAB
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