Manufactured by:BT LAB
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Human Epithelial Neutrophil Activating Peptide 78, ENA-78 ELISA Kit

Human Epithelial Neutrophil Activating Peptide 78, ENA-78 ELISA Kit

SKU:
ABM207778
Manufacturer SKU
E0131Hu
Product type
ELISA Kits
TargetCXCL5
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CXCL5 antibody. CXCL5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CXCL5 Antibody is added and binds to CXCL5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CXCL5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CXCL5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
C-X-C motif chemokine 5;CXCL 5;CXCL5;ENA-78(1-78;ENA-78(8-78;ENA-78(9-78;Epithelial-derived neutrophil-activating protein 78;Neutrophil-activating Peptide ENA-78;Small-inducible cytokine B5;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.16ng/mL
Detection range
0.3-90ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
11,972 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0131Hu
Gene symbol
CXCL5
Gene ID
6374
UniProt accession
P42830
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CXCL5C-X-C motif chemokine ligand 5

View target guide

CXCL5 identifies C-X-C motif chemokine ligand 5 in Homo sapiens (Human). This target record connects NCBI Gene 6374, UniProtKB P42830 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6374
UniProtKB
P42830
BT LAB
£344.83£293.00