Manufactured by:BT LAB
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Porcine Interleukin 15, IL-15 ELISA Kit

Porcine Interleukin 15, IL-15 ELISA Kit

SKU:
ABM207754
Manufacturer SKU
E0124Po
Product type
ELISA Kits
TargetIL15
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA (By similarity). In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IL15 antibody. IL15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IL15 Antibody is added and binds to IL15 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL15 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IL15. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
IL 15;IL15;IL-15;Interleukin-15
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.152ng/L
Detection range
0.3-90ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
18,437 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0124Po
Gene symbol
IL15
Gene ID
397683
UniProt accession
Q95253
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL15interleukin 15

View target guide

IL15 identifies interleukin 15 in Homo sapiens (Human). This target record connects NCBI Gene 3600, UniProtKB P40933 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3600
UniProtKB
P40933
BT LAB
£240.93£205.00