Manufactured by:BT LAB
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Sheep Interleukin 5, IL-5 ELISA Kit

Sheep Interleukin 5, IL-5 ELISA Kit

SKU:
ABM207420
Manufacturer SKU
E0052Sh
Product type
ELISA Kits
TargetIL-5
ApplicationsELISA
Species / reactivitySheep
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Sheep IL-5 antibody. IL-5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep IL-5 Antibody is added and binds to IL-5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Sheep IL-5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
B-cell growth factor II;BCGF-II;Cytotoxic T-lymphocyte inducer;Eosinophil differentiation factor;IL 5;IL5;IL-5;Interleukin-5;T-cell replacing factor;TRF
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
4.51ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
14,974 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0052Sh
Gene symbol
IL5
Gene ID
443350
UniProt accession
Q28586
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL5interleukin 5

View target guide

IL5 identifies interleukin 5 in Homo sapiens (Human). This target record connects NCBI Gene 3567, UniProtKB P05113 and related ABMIUM catalogue products.

Biological function: IL5 is a homodimeric cytokine that supports eosinophil survival, differentiation and chemotaxis and can promote immunoglobulin production, growth and differentiation in B cells.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3567
UniProtKB
P05113
BT LAB
£240.93£205.00