Manufactured by:BT LAB
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Bovine Serum amyloid A, SAA ELISA Kit

Bovine Serum amyloid A, SAA ELISA Kit

SKU:
ABM207260
Manufacturer SKU
E0023Bo
Product type
ELISA Kits
TargetSAA
ApplicationsELISA
Species / reactivityBovine
Sample typeSerum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue, Cell culture supernatants, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine SAA antibody. SAA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine SAA Antibody is added and binds to SAA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SAA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine SAA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
SAA1;SAA
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.054ug/ml
Detection range
0.1-40ug/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
14,516 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaUrineAscitesCerebrospinal fluidTissueCell culture supernatantsTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0023Bo
Gene symbol
SAA1
Gene ID
506412
UniProt accession
P35541
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8°C
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SAA1serum amyloid A1

View target guide

SAA1 identifies serum amyloid A1 in Homo sapiens (Human). This target record connects NCBI Gene 6288, UniProtKB P0DJI8 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6288
UniProtKB
P0DJI8
BT LAB
£240.93£205.00