Manufactured by:BT LAB
Save 15%RUO

Canine Vascular Cell Adhesion Molecule 1, VCAM-1 ELISA Kit

Canine Vascular Cell Adhesion Molecule 1, VCAM-1 ELISA Kit

SKU:
ABM207244
Manufacturer SKU
E0020Ca
Product type
ELISA Kits
TargetVCAM1
ApplicationsELISA
Species / reactivityCanine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine VCAM1 antibody. VCAM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine VCAM1 Antibody is added and binds to VCAM1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VCAM1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine VCAM1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cancer
Also known as
CD antigen CD106;Vascular cell adhesion protein 1;VCAM 1;V-CAM 1;VCAM1;VCAM-1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.24ng/ml
Detection range
2-600ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
81,412 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0020Ca
Gene symbol
VCAM1
Gene ID
403982
UniProt accession
Q28260
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VCAM1vascular cell adhesion molecule 1

View target guide

VCAM1 identifies vascular cell adhesion molecule 1 in Homo sapiens (Human). This target record connects NCBI Gene 7412, UniProtKB P19320 and related ABMIUM catalogue products.

Biological function: VCAM1 is an endothelial cell-adhesion glycoprotein that regulates leukocyte adhesion and transendothelial migration through interactions with leukocyte integrins during immune surveillance and inflammation.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7412
UniProtKB
P19320
BT LAB
£357.63£304.00