Manufactured by:BT LAB
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Rabbit C-Reactive Protein, CRP ELISA Kit

Rabbit C-Reactive Protein, CRP ELISA Kit

SKU:
ABM207222
Manufacturer SKU
E0016Rb
Product type
ELISA Kits
TargetCRP
ApplicationsELISA
Species / reactivityRabbit
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit CRP antibody. CRP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit CRP Antibody is added and binds to CRP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CRP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit CRP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
C-reactive protein;CRP
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.91ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
25,493 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0016Rb
Gene symbol
CRP
Gene ID
100009091
UniProt accession
P02742
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CRPC-reactive protein

View target guide

CRP identifies C-reactive protein in Homo sapiens (Human). This target record connects NCBI Gene 1401, UniProtKB P02741 and related ABMIUM catalogue products.

Biological function: CRP is a pentraxin acute-phase protein that binds phosphocholine in a calcium-dependent manner and participates in innate host defence, including complement-associated and opsonisation-related responses.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1401
UniProtKB
P02741
BT LAB
£240.93£205.00