AF (Total Aflatoxin) ELISA Kit

Representative Elabscience ELISA kit packaging; contents vary by catalogue and size

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Manufactured by:Elabscience
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AF (Total Aflatoxin) ELISA Kit

Quantitative total aflatoxin ELISA with a 1.5 ppb detection limit.

SKU:
ABM150534-1
Manufacturer SKU
E-TO-E032
Product type
ELISA Kits
TargetAF
ApplicationsELISA
Species / reactivityUniversal
Sample typeGrain, cereals, feed, feed raw materials, peanut meal, vegetable oil

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

AF (Total Aflatoxin) ELISA Kit from Elabscience is intended for quantitative analysis of total aflatoxin residues in grain, cereals, feed, feed raw materials, peanut meal and vegetable oil.

Aflatoxins are mycotoxins produced by Aspergillus flavus and Aspergillus parasiticus. They can occur in food, feed and agricultural raw materials.

The competitive, colourimetric ELISA uses a 96-well microtiter plate (12 strips of 8 wells). The supplier lists a detection limit of 1.5 ppb for the documented matrices and an incubation time of 30 minutes. Consult the manual for extraction instructions and matrix-specific limitations. Research use only.

Test principle

This kit uses Competitive-ELISA as the method for the quantitative detection. It can detect Total Aflatoxin (AF) in samples, such as grain, feed, edible oil, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard liquid and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.
Key facts & specifications
Research areas
Metabolism
Also known as
Total Aflatoxin
Incubation temperature
25 ± 2°C
Assay type
ELISA
Detection method
Colourimetric competitive ELISA
Sensitivity
1.5 ppb
Plate format
96-well microtiter plate; 12 strips of 8 wells
Wells / tests
96T / 96T*3 / 96T*5
Assay duration
30 minutes
Form / buffer
ELISA kit
Quantity per pack
96T / 96T*3 / 96T*5
Technical note
Matrix-specific LOD: grain, cereals, feed and feed raw materials — 1.5 ppb; peanut meal and vegetable oil — 1.5 ppb (see supplementary instructions). Source manual also lists coffee beans at 1.5 ppb; this is retained as manual evidence, without extending the listed validation matrix. Assay reaction conditions: 25 ± 2°C. Standard concentrations: 0, 1, 3, 9, 27 and 81 ppb; these are calibration points, not a claimed linear detection range.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Evidence status by application and model.

EvidenceGraincerealsfeedfeed raw materialspeanut mealvegetable oil
ABMIUM Verified
Identifiers & database references
Manufacturer SKU
E-TO-E032
Storage, stability & shipping
Shelf life
12 months from production; follow the labelled expiry date
Storage conditions
2-8℃, do not freeze, 11-12 months
Short-term storage
2-8℃, do not freeze, 11-12 months
Long-term storage
2–8°C; do not freeze. Supplier-listed shelf life: 11–12 months.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£227.00