AFB1(Aflatoxin B1) ELISA Kit

Representative Elabscience ELISA kit packaging; contents vary by catalogue and size

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Manufactured by:Elabscience
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AFB1(Aflatoxin B1) ELISA Kit

AFB1(Aflatoxin B1) ELISA Kit from Elabscience.

SKU:
ABM150522-1
Manufacturer SKU
E-TO-E017
Product type
ELISA Kits
TargetAFB1
ApplicationsELISA
Species / reactivityUniversal
Sample typeFinished feed, meals, grains, seasonings, biscuits, edible oil, beef, pork, ham sausages, modified milk

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

AFB1(Aflatoxin B1) ELISA Kit is supplied by Elabscience as an ELISA kit for quantitative analysis. Supplier-listed sample types: Finished feed, meals, grains, seasonings, biscuits, edible oil, beef, pork, ham sausages, modified milk.

Aflatoxin B1 is the most common substance in food and one of the most genotoxic and carcinogenic aflatoxin. Generally speaking, aflatoxin b1 exists in many natural foods, such as corn, peanuts, edible oil, beans, grains, etc. It is hard to dissolve in water and heat resistant, so it is difficult to be destroyed under normal cooking and processing temperatures.

Detection method: Colorimetric method;ELISA;Competitive. Supplier-listed detection limit: 3 ppb; 1 ppb; 0.5 ppb; 0.1 ppb (matrix-dependent). Incubation time: 45 min. Format: Microtiter plate with 96 wells (12 strips with 8 wells each).

Available pack sizes: 96T / 96T*3 / 96T*5. Refer to the manufacturer instructions for sample preparation and assay conditions. Research use only.

Test principle

This kit uses Competitive-ELISA as the method for the quantitative detection. Itcan detect Aflatoxin B1 (AFB1) in samples, such as grain, feed, edible oil,etc. This kit iscomposed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard liquid and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, AFB1 in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AFB1 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There isa negative correlation between the OD value of samples and the concentration of AFB1. The concentration of AFB1 in the samples can be calculated by comparing the OD of the samples to the standard curve.
Key facts & specifications
Research areas
Metabolism
Also known as
AFB1;Aflatoxin B1
Assay type
ELISA
Detection method
Colorimetric method;ELISA;Competitive
Sensitivity
3 ppb; 1 ppb; 0.5 ppb; 0.1 ppb (matrix-dependent)
Detection range
See the manual for details
Plate format
Microtiter plate with 96 wells (12 strips with 8 wells each).
Wells / tests
96T / 96T*3 / 96T*5
Assay duration
45 min
Form / buffer
ELISA kit
Quantity per pack
96T / 96T*3 / 96T*5
Technical note
Matrix-specific supplier sensitivity / detection limits: Finished feed (swine, bovine), meals (soybean meal, rapeseed meal), cereals (corn flour, corn bran, wheat bran, soybean flour, mung bean flour, red bean flour, rice flour, barley flour, sorghum flour, wheat flour, plain flour, peanut), seasonings (chili powder, cumin powder, soy sauce, fermented black soybean, star anise powder, sichuan pepper powder, black pepper powder), biscuits---3 ppb; Edible oil---1 ppb; Beef, pork, ham sausage---0.5 ppb; Modified milk (grain milk, wheat-flavored breakfast milk)---0.1 ppb. Detection instrument: Microtiter plate spectrophotometer (450 nm/630nm) Principle source: https://789.bio/ea/nbnHyT#page=2 Lead-time discrepancy: supplied price list states 2-3 business days; manufacturer variant records state 4-6 days. Confirm dispatch timing when ordering.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Evidence status by application and model.

EvidenceFinished feedmealsgrainsseasoningsbiscuitsedible oilbeefporkham sausagesmodified milk
ABMIUM Verified
Identifiers & database references
Manufacturer SKU
E-TO-E017
Storage, stability & shipping
Shelf life
12 months from production; follow the labelled expiry date
Storage conditions
2-8℃
Short-term storage
2-8℃
Long-term storage
2-8℃
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£265.00