Human Toxoplasma (TOX) IgG Avidity ELISA Kit

This ELISA kit uses Indirect-ELISA as the principle to detect the TOX-IgG Avidity in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with purified TOX-antigen. Samples are added to the ELISA Microtiter plate wells and the TOX antibody in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The control solution and the dissociation solution is added to each well. After the incubation reaction, the low-affinity Toxoplasma IgG antibodies were separated from the antigen under the action of the dissociation buffer. Free components are washed away. The HRP conjugate is added to each well and react with the compound to form “TOX antigen-TOX antibody-HRP antibody” compound, the substrate reagent is added to initiatethe color developing reaction. After colorimetry on the microplate reader, the avidity index (AI) of the sample to be tested is calculated based on the ratio of the absorbance of the dissociation well to that of the parallel control well.