Manufactured by:Elabscience
ABMIUM Verified™RUO

MEL(Melamine) ELISA Kit

MEL(Melamine) ELISA Kit from Elabscience.

SKU:
ABM150220-1
Manufacturer SKU
E-FS-E010
Product type
ELISA Kits
TargetMEL
ApplicationsELISA
Species / reactivityUniversal
Sample typeMilk powder, milk, tissues (chicken/pig/duck/fish/shrimp/liver), feed, eggs, serum

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

MEL(Melamine) ELISA Kit is supplied by Elabscience as an ELISA kit for quantitative analysis. Supplier-listed sample types: Milk powder, milk, tissues (chicken/pig/duck/fish/shrimp/liver), feed, eggs, serum.

Melamine is a chemical substance used in a variety of industrial production and is used illegally to increase the apparent protein content of milk and other foods. Trace amounts of melamine can contaminate food by contact without causing health problems, however it is toxic at high levels.

Detection method: Colorimetric method;ELISA;Competitive. Supplier-listed detection limit: 40 ppb; 54 ppb; 2 ppb; 4 ppb; 200 ppb; 8 ppb (matrix-dependent). Incubation time: 45 minutes. Format: Microtiter plate with 96 wells (12 strips with 8 wells each).

Available pack sizes: 96T / 96T*3 / 96T*5. Refer to the manufacturer instructions for sample preparation and assay conditions. Research use only.

Test principle

This kit uses Competitive-ELISA as the method for the quantitative detection. Itcan detect Melamine (MEL) in samples, such as milk, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, MEL in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-MEL antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of MEL. The concentration of MEL in the samples can be calculated by comparing the OD of the samples to the standard curve.
Key facts & specifications
Research areas
Metabolism
Also known as
Melamine
Assay type
ELISA
Detection method
Colorimetric method;ELISA;Competitive
Sensitivity
40 ppb; 54 ppb; 2 ppb; 4 ppb; 200 ppb; 8 ppb (matrix-dependent)
Detection range
See the manual for details
Plate format
Microtiter plate with 96 wells (12 strips with 8 wells each).
Wells / tests
96T / 96T*3 / 96T*5
Assay duration
45 minutes
Form / buffer
ELISA kit
Quantity per pack
96T / 96T*3 / 96T*5
Technical note
Matrix-specific supplier sensitivity / detection limits: Milk powder---40 ppb; milk---54 ppb; milk/milk powder (Method 2)---2 ppb; tissue (chicken/pork/duck/fish/shrimp/liver)---4 ppb; feed---200 ppb; eggs---40 ppb; serum---8 ppb. Detection instrument: Microtiter plate spectrophotometer (450 nm/630nm) Principle source: https://789.bio/ea/eT8uLG#page=2 Lead-time discrepancy: supplied price list states 2-3 business days; manufacturer variant records state 4-6 days. Confirm dispatch timing when ordering.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Evidence status by application and model.

EvidenceMilk powdermilktissues (chicken/pig/duck/fish/shrimp/liver)feedeggsserum
ABMIUM Verified
Identifiers & database references
Manufacturer SKU
E-FS-E010
Storage, stability & shipping
Shelf life
12 months from production; follow the labelled expiry date
Storage conditions
2-8℃, avoid freeze, 11-12 months
Short-term storage
2-8℃, avoid freeze, 11-12 months
Long-term storage
2-8℃, avoid freeze, 11-12 months
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MAP2K1mitogen-activated protein kinase kinase 1

View target guide

MAP2K1 identifies mitogen-activated protein kinase kinase 1 in Homo sapiens (Human). This target record connects NCBI Gene 5604, UniProtKB Q02750 and related ABMIUM catalogue products.

Biological function: Dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1; MEK1/MKK1) is a dual-specificity kinase in the RAS-RAF-MEK-ERK pathway. It phosphorylates and activates ERK1/ERK2, coupling upstream growth-factor signals to cellular proliferation, differentiation and survival programs.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5604
UniProtKB
Q02750
Elabscience
£227.00