Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM100359

3MG rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

3MG rabbit pAb from ELK Biotechnology Read more ›

3MG rabbit pAb
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3MG rabbit pAb

Product Description

3MG rabbit pAb is a research-use antibody from ELK Biotechnology for studying 3MG in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

alternative products:Experimental confirmation may be lacking for some isoforms,catalytic activity:Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.,function:Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.,similarity:Belongs to the DNA glycosylase MPG family.,subunit:Binds MBD1.,

Additional antibody information

Specificity 3MG Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: 3MG
Collaborator ELK Biotechnology
Partner model ES9599
Target 3MG
Research areas>>Base excision repair
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Epithelium,Liver,Pancreas,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
3MG
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
32kD
Cellular localization
Cytoplasm . Mitochondrion matrix, mitochondrion nucleoid . Nucleus .
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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