14-3-3 ζ/δ rabbit pAb from ELK Biotechnology Read more ›
14-3-3 ζ/δ rabbit pAb is a research-use antibody from ELK Biotechnology for studying 14-3-3 ζ/δ in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
| Specificity | 14-3-3 ζ/δ Polyclonal Antibody detects endogenous levels of 14-3-3 ζ/δ protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of NIH/3T3 cells, using 14-3-3 zeta/delta Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using 14-3-3 zeta/delta Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 cells, using 14-3-3 zeta/delta Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7525 |
| Target | 14-3-3 ζ/δ |
| Research areas | >>Cell cycle, >>Oocyte meiosis, >>PI3K-Akt signaling pathway, >>Hippo signaling pathway, >>Hepatitis C, >>Hepatitis B, >>Viral carcinogenesis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta/delta. AA range:196-245 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B-cell lymphoma,Bone marrow |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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