14-3-3σ (Phospho Ser186) rabbit pAb from ELK Biotechnology Read more ›
14-3-3σ (Phospho Ser186) rabbit pAb is a research-use antibody from ELK Biotechnology for studying 14-3-3σ in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.,function:p53-regulated inhibitor of G2/M progression.,similarity:Belongs to the 14-3-3 family.,subcellular location:May be secreted by a non-classical secretory pathway.,subunit:Homodimer. Interacts with KRT17 (By similarity). Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN.,tissue specificity:Present mainly in tissues enriched in stratified squamous keratinising epithelium.,
| Specificity | This antibody detects endogenous levels of Human,Mouse 14-3-3σ (Phospho Ser186) |
|---|---|
| Validation evidence captions | Western Blot analysis of 1 A431 cell 2 LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES20196 |
| Target | 14-3-3σ |
| Research areas | >>Cell cycle, >>p53 signaling pathway, >>Aldosterone-regulated sodium reabsorption |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1000-2000 ELISA 1:5000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human 14-3-3σ (Phospho Ser186) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Present mainly in tissues enriched in stratified squamous keratinizing epithelium. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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