12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit from ELK Biotechnology Read more ›
12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring 12-HETE in Serum, Plasma, Other biological fluids samples from General. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include 12HETE.
| Collaborator | ELK Biotechnology |
| Partner model | ELK8156 |
| Target | 12-HETE |
| Research areas | Signal transduction, Metabolic pathway, Infection immunity, Hormone metabolism, Dermatology |
| Reactivity | General |
| Applications | ELISA |
| Assay type | Competitive Inhibition |
| Sample types | Serum,Plasma,Other biological fluids |
| Detection range | 15.63-1000 ng/mL |
| Sensitivity | 5.13 ng/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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