Recombinant Human CD32a/FCGR2A Protein (167 His, His&AVI Tag), Biotinylated
Recombinant Human CD32a/FCGR2A Protein (167 His, His&AVI Tag), Biotinylated supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. 3. Labeling ratio of biotin to protein: 0.1-0.6. ,33 kDa"]Product Description
CD32a/FCGR2A is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,CD32a/FCGR2A ,Recombinant Proteins; predicted molecular weight: 23.6 kDa.
Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. 3. Labeling ratio of biotin to protein: 0.1-0.6.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 23.6 kDa.
Database references: UniProt: P12318-1 | NCBI Gene: 2212 | Gene symbol: CD32a/FCGR2A.
Research Use Only.
Key Facts
| Target | CD32a/FCGR2A |
| Also known as | CD32, CD32A, CDw32, FCG2, FCGR2 , FCGR2A, FCGR2A1, Fc gamma RIIA, Fc-gamma RII-a, Fc-gamma-RIIa, FcGR, FcRII-a, IGFR2, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a |
| Species reactivity | Human |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Immunology, Stem Cells |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.