Collaborator Elabscience
Recombinant ProteinsRecombinantISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-15512

Recombinant Human CD32a/FCGR2A Protein (167 His, His&AVI Tag), Biotinylated

1. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml. 2. Using the Octet RED Systemthe affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. 3. Labeling ratio of biotin to protein: 0.1-0.6.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human CD32a/FCGR2A Protein (167 His, His&AVI Tag), Biotinylated supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Loaded Biotinylated Human FcγRIIA / CD32a (H167) recombinant protein (His & Avi Tag) (Cat. PKSH030290) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 0.53 μM as determined in a BLI assay (Sartorius Octet Red384).
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Loaded Biotinylated Human FcγRIIA / CD32a (H167) recombinant protein (His & Avi Tag) (Cat. PKSH030290) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 0.53 μM as determined in a BLI assay (Sartorius Octet Red384).["1. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. 3. Labeling ratio of biotin to protein: 0.1-0.6. ,33 kDa"]

Product Description

CD32a/FCGR2A is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,CD32a/FCGR2A ,Recombinant Proteins; predicted molecular weight: 23.6 kDa.

Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: 1. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. 3. Labeling ratio of biotin to protein: 0.1-0.6.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 23.6 kDa.

Database references: UniProt: P12318-1 | NCBI Gene: 2212 | Gene symbol: CD32a/FCGR2A.

Research Use Only.

Key Facts

Product typeRecombinant Proteins Target: CD32a/FCGR2A
Collaborator Elabscience
Partner model PKSH030290_100ug
Target CD32a/FCGR2A
Research areasImmunology, Stem Cells
Reactivity Human
Purity >95% by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Shelf life 12 months
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryBioactivityBindingCell-BasedIn VivoELISA Capture
Human

Specifications

Target
CD32a/FCGR2A
Also known as
CD32, CD32A, CDw32, FCG2, FCGR2 , FCGR2A, FCGR2A1, Fc gamma RIIA, Fc-gamma RII-a, Fc-gamma-RIIa, FcGR, FcRII-a, IGFR2, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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